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dc.contributor.authorNelissen, Inge
dc.contributor.authorHaase, Andrea
dc.contributor.authorAnguissola, Sergio
dc.contributor.authorRocks, Louise
dc.contributor.authorJacobs, An
dc.contributor.authorWillems, Hanny
dc.contributor.authorRiebeling, Christian
dc.contributor.authorLuch, Andreas
dc.contributor.authorPiret, Jean-Pascal
dc.contributor.authorToussaint, Olivier
dc.contributor.authorTrouiller, Bénédicte
dc.contributor.authorLacroix, Ghislaine
dc.contributor.authorGutleb, Arno C.
dc.contributor.authorContal, Servane
dc.contributor.authorDiabaté, Silvia
dc.contributor.authorWeiss, Carsten
dc.contributor.authorLozano-Fernández, Tamara
dc.contributor.authorGonzález-Fernández, África
dc.contributor.authorDušinská, Mária
dc.contributor.authorHuk, Anna
dc.contributor.authorStone, Vicki
dc.contributor.authorKanase, Nilesh
dc.contributor.authorNocuń, Marek
dc.contributor.authorStępnik, Maciej
dc.contributor.authorMeschini, Stefania
dc.contributor.authorAmmendolia, Maria Grazia
dc.contributor.authorLewinski, Nastassja A.
dc.contributor.authorRiediker, Michael
dc.contributor.authorVenturini, Marco
dc.contributor.authorBenetti, Federico
dc.contributor.authorTopinka, Jan
dc.contributor.authorBrzicová, Táňa
dc.contributor.authorMilani, Silvia
dc.contributor.authorRädler, Joachim O.
dc.contributor.authorSalvati, Anna
dc.contributor.authorDawson, Kenneth A.
dc.date.accessioned2020-10-25T10:39:45Z
dc.date.available2020-10-25T10:39:45Z
dc.date.issued2020
dc.identifier.citationNanomaterials. 2020, vol. 10, issue 8, art. no. 1430.cs
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/10084/142359
dc.description.abstractThe quality and relevance of nanosafety studies constitute major challenges to ensure their key role as a supporting tool in sustainable innovation, and subsequent competitive economic advantage. However, the number of apparently contradictory and inconclusive research results has increased in the past few years, indicating the need to introduce harmonized protocols and good practices in the nanosafety research community. Therefore, we aimed to evaluate if best-practice training and inter-laboratory comparison (ILC) of performance of the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay for the cytotoxicity assessment of nanomaterials among 15 European laboratories can improve quality in nanosafety testing. We used two well-described model nanoparticles, 40-nm carboxylated polystyrene (PS-COOH) and 50-nm amino-modified polystyrene (PS-NH2). We followed a tiered approach using well-developed standard operating procedures (SOPs) and sharing the same cells, serum and nanoparticles. We started with determination of the cell growth rate (tier 1), followed by a method transfer phase, in which all laboratories performed the first ILC on the MTS assay (tier 2). Based on the outcome of tier 2 and a survey of laboratory practices, specific training was organized, and the MTS assay SOP was refined. This led to largely improved intra- and inter-laboratory reproducibility in tier 3. In addition, we confirmed that PS-COOH and PS-NH2 are suitable negative and positive control nanoparticles, respectively, to evaluate impact of nanomaterials on cell viability using the MTS assay. Overall, we have demonstrated that the tiered process followed here, with the use of SOPs and representative control nanomaterials, is necessary and makes it possible to achieve good inter-laboratory reproducibility, and therefore high-quality nanotoxicological data.cs
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofseriesNanomaterialscs
dc.relation.urihttp://doi.org/10.3390/nano10081430cs
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectnanosafetycs
dc.subjectcytotoxicitycs
dc.subjectinter-laboratory comparisoncs
dc.subjectbest practicecs
dc.subjecttrainingcs
dc.titleImproving quality in nanoparticle-induced cytotoxicity testing by a tiered inter-laboratory comparison studycs
dc.typearticlecs
dc.identifier.doi10.3390/nano10081430
dc.rights.accessopenAccesscs
dc.type.versionpublishedVersioncs
dc.type.statusPeer-reviewedcs
dc.description.sourceWeb of Sciencecs
dc.description.volume10cs
dc.description.issue8cs
dc.description.firstpageart. no. 1430cs
dc.identifier.wos000567292700001


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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.