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dc.contributor.authorBrzicová, Táňa
dc.contributor.authorJavorková, Eliška
dc.contributor.authorVrbová, Kristýna
dc.contributor.authorZajícová, Alena
dc.contributor.authorHolan, Vladimír
dc.contributor.authorPinkas, Dominik
dc.contributor.authorPhilimonenko, Vlada
dc.contributor.authorSikorová, Jitka
dc.contributor.authorKléma, Jiří
dc.contributor.authorTopinka, Jan
dc.contributor.authorRössner Jr., Pavel
dc.date.accessioned2019-10-22T05:06:18Z
dc.date.available2019-10-22T05:06:18Z
dc.date.issued2019
dc.identifier.citationNanomaterials. 2019, vol. 9, issue 5, art. no. 687.cs
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/10084/138877
dc.description.abstractIn the body, engineered nanoparticles (NPs) may be recognized and processed by immune cells, among which macrophages play a crucial role. We evaluated the effects of selected NPs [NM-100 (TiO2), NM-110 (ZnO), NM-200 (SiO2), and NM-300 K (Ag)] on THP-1 macrophage-like cells. The cells were exposed to subcytotoxic concentrations of NPs (1-25 mu g/mL) and the expression of immunologically relevant genes (VCAM1, TNFA, CXCL8, ICAM1, CD86, CD192, and IL1B) was analyzed by RT-qPCR. The expression of selected cytokines, growth factors and surface molecules was assessed by flow cytometry or ELISA. Generation of reactive oxygen species and induction of DNA breaks were also analyzed. Exposure to diverse NPs caused substantially different molecular responses. No significant effects were detected for NM-100 treatment. NM-200 induced production of IL-8, a potent attractor and activator of neutrophils, growth factors (VEGF and IGF-1) and superoxide. NM-110 triggered a proinflammatory response, characterized by the activation of transcription factor NF-kappa B, an enhanced production of proinflammatory cytokines (TNF-alpha) and chemokines (IL-8). Furthermore, the expression of cell adhesion molecules VCAM-1 and ICAM-1 and hepatocyte growth factor (HGF), as well as superoxide production and DNA breaks, were affected. NM-300 K enhanced IL-8 production and induced DNA breaks, however, it decreased the expression of chemokine receptor (CCR2) and CD86 molecule, indicating potential immunosuppressive activity. The toxicity of ZnO and Ag NPs was probably caused by their intracellular dissolution, as indicated by transmission electron microscopy imaging. The observed effects in macrophages might further influence both innate and adaptive immune responses by promoting neutrophil recruitment via IL-8 release and enhancing the adhesion and stimulation of T cells by VCAM-1 and ICAM-1 expression.cs
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofseriesNanomaterialscs
dc.relation.urihttp://doi.org/10.3390/nano9050687cs
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectnanoparticlescs
dc.subjectTHP-1 cellscs
dc.subjectimmune responsecs
dc.subjectreactive oxygen speciescs
dc.subjectDNA damagecs
dc.titleMolecular responses in THP-1 macrophage-like cells exposed to diverse nanoparticlescs
dc.typearticlecs
dc.identifier.doi10.3390/nano9050687
dc.rights.accessopenAccesscs
dc.type.versionpublishedVersioncs
dc.type.statusPeer-reviewedcs
dc.description.sourceWeb of Sciencecs
dc.description.volume9cs
dc.description.issue5cs
dc.description.firstpageart. no. 687cs
dc.identifier.wos000479007900029


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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Except where otherwise noted, this item's license is described as © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.