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dc.contributor.authorValuchová, Soňa
dc.contributor.authorMikulková, Pavlína
dc.contributor.authorPečinková, Jana
dc.contributor.authorKlímová, Jana
dc.contributor.authorKrumnikl, Michal
dc.contributor.authorBainar, Petr
dc.contributor.authorHeckmann, Stefan
dc.contributor.authorTomančák, Pavel
dc.contributor.authorŘíha, Karel
dc.date.accessioned2020-03-25T22:59:23Z
dc.date.available2020-03-25T22:59:23Z
dc.date.issued2020
dc.identifier.citationeLife. 2020, vol. 9 , art. no. e52546.cs
dc.identifier.issn2050-084X
dc.identifier.urihttp://hdl.handle.net/10084/139362
dc.description.abstractIn higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.cs
dc.language.isoencs
dc.publishereLife Sciences Publications Ltdcs
dc.relation.ispartofserieseLifecs
dc.relation.urihttps://doi.org/10.7554/eLife.52546cs
dc.rightsCopyright Valuchova et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.titleImaging plant germline differentiation within Arabidopsis flowers by light sheet microscopycs
dc.typearticlecs
dc.identifier.doi10.7554/eLife.52546
dc.rights.accessopenAccesscs
dc.type.versionpublishedVersioncs
dc.type.statusPeer-reviewedcs
dc.description.sourceWeb of Sciencecs
dc.description.volume9cs
dc.description.firstpageart. no. e52546cs
dc.identifier.wos000514104400001


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Copyright Valuchova et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Except where otherwise noted, this item's license is described as Copyright Valuchova et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.